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Autoradiographic study of hyphal cell wall synthesis of Saprolegnia
Authors:Michel Fèvre  Mireille Rougier
Institution:(1) Laboratoire de Physiologie Végétale, L. A. CNRS no. 44, Univerté Claude Bernard, F-69622 Villeurbanne Cédex, France;(2) Laboratoire d'Histophysiology de la Sécrétation, CMEAB, Université Claude Bernard, F-69622 Villeurbanne Cédex, France
Abstract:Incorporation of radioactive glucose in growing apices of Saprolegnia monoïca hyphae were examined by electron microscopic autoradiography. 3H glucose labelling indicates that dictyosomes and apical vesicles do not contain much polysaccharide and that glucan synthesis occurs at the cell surface. 14C glucose labelling shows that incorporation was chased from the cell walls during hyphal morphogenesis.
Keywords:Autoradiography  Apical vesicles  Glucose labelling  Saprolegnia  Cell wall synthesis
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