Cloning, sequencing and characterization of the Saccharomyces cerevisiae URA7 gene encoding CTP synthetase |
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Authors: | Odile Ozier-Kalogeropoulos Franco Fasiolo Marie-Therèse Adeline Jocelyne Collin Fran?ois Lacroute |
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Affiliation: | (1) Centre de Génétique Moléculaire du C.N.R.S., Laboratoire propre associé à l'Université Pierre et Marie Curie, F-91198 Gif-sur-Yvette Cedex, France;(2) I.B.M.C. du C.N.R.S., 15 rue R. Descartes, F-67084 Strasbourg Cedex, France;(3) Institut de Chimie des Substances Naturelles du C.N.R.S., F-91198 Gif-sur-Yvette Cedex, France |
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Abstract: | Summary The URA7 gene of Saccharomyces cerevisiae encodes CTP synthetase (EC 6.3.4.2) which catalyses the conversion of uridine 5 -triphosphate to cytidine 5 -triphosphate, the last step of the pyrimidine biosynthetic pathway. We have cloned and sequenced the URA 7 gene. The coding region is 1710 by long and the deduced protein sequence shows a strong degree of homology with bacterial and human CTP synthetases. Gene disruption shows that URA7 is not an essential gene: the level of the intracellular CTP pool is roughly the same in the deleted and the wild-type strains, suggesting that an alternative pathway for CTP synthesis exists in yeast. This could involve either a divergent duplicated gene or a different route beginning with the amination of uridine mono- or diphosphate. |
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Keywords: | Saccharomyces cerevisiae CTP synthetase Pyrimidine pathway Intracellular pyrimidine pool Non-essential gene |
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