An effective method for the in situ synthesis of DNA-CPG conjugates using chemical ligation technology as tools for SNP analysis |
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Authors: | Ohkubo Akihiro Tanaka Kunihiko Taguchi Haruhiko Seio Kohji Nagasawa Hiroshi Tsukahara Toshifumi Sekine Mitsuo |
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Institution: | 4259, Nagatsuta, Department of Life Science, Tokyo Institute of Technology, Yokohama 226-8501, Japan. |
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Abstract: | In this paper, we report a new method for the SNP analysis by using a chemical ligation (CL) technique on CPG plates with high coupling efficiency. This method showed markedly high match/mismatch discrimination ability. Particularly, replacement of thymidine with 2-thiothymidine in DNA probes used in the CL technology resulted in significant improvement of the base discrimination ability of the thymine base in this system. |
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