Lipase production of Staphylococcus carnosus in a dialysis fermentor |
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Authors: | Max Lechner Herbert Märkl Friedrich Götz |
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Institution: | (1) Lehrstuhl für Mikrobiologie, Technische Universität München, Arcisstrasse 21, D-8000 Munich 2, Federal Republic of Germany;(2) Arbeitsbereich Bioprozess-und Bioverfahrenstechnik, Technische Universität Hamburg-Harburg, Harburger Schlosstrasse 37, D-2000, Hamburg 90, Federal Republic of Germany;(3) Mikrobielle Genetik, Universität Tübingen, Auf der Morgenstelle 28, D-7400 Tübingen 1, Federal Republic of Germany |
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Abstract: | Summary In a newly constructed one-vessel dialysis fermentor, a strain of Staphylococcus carnosus TM300 carrying the lipase secretion plasmid pLipPS1 was used to investigate exoenzyme and biomass production. The bacterial culture grows in an inner compartment of 21 volume, separated from a 101 nutrient broth compartment by a conventional dialysis membrane. In order to avoid substrate depletion and to prolong the growth phase, a highly concentrated nutrient broth was used. The biomass production reached 60 g cell dry weight/l. The increase in extracellular lipase concentration was directly coupled with the increase of cell mass and reached a value of 230 mg/l culture supernatant. Harvesting the cells in the late growth phase, the lipase content was about 30% of the total exoproteins in the supernatant. |
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