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Invertase in Immobilized Cells of Eschscholtzia Californica |
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| Authors: | K Neubert J Stano K Mičieta P Kovács H Tintemann |
| Institution: | (1) Institute of Biochemistry, M. Luther University, K. Mothes Str. 3, D-06120 Halle, Germany;(2) Faculty of Pharmacy, Comenius University, Odbojárov 10, SK-83232 Bratislava, Slovak Republic;(3) Faculty of Natural Sciences, Comenius University, Révová 39, SK-81102 Bratislava, Slovak Republic |
| Abstract: | Cell suspension culture of Eschscholtzia californica Cham. were permeabilized by Tween 20 or 80, and immobilized by glutaraldehyde. The highest invertase activity was at pH 4.5 and temperature 50 °C. The hydrolysis of the substrate was linear for 5 h reaching 60 % conversion. The cells had high invertase activity and a good stability, and in long-term storage they showed convenient physico-mechanical properties. |
| Keywords: | Californian poppy cell permeabilization glutaraldehyde |
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