Gravity and light control of the developmental polarity of regenerating protoplasts isolated from prothallial cells of the fern Ceratopteris richardii |
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Authors: | E S Edwards S J Roux |
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Institution: | (1) Department of Botany, The University of Texas at Austin, Austin, TX 78713, USA Fax no.: +1+512-471-3878 E-mail: sroux@uts.cc.utexas.edu, US |
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Abstract: | A procedure has been developed for isolating protoplasts from prothalli of Ceratopteris richardii which can be cultured and are capable of regeneration. Protoplasts were isolated from 2-week-old gametophytes in a medium
containing wall-digesting enzymes in 0.5 m sucrose, followed by purification of the released protoplasts by floating them up into a 0.5 m sorbitol layer. Regeneration occurred over a period of 10–24 days, and, under optimal osmotic conditions, followed the developmental
pattern seen during spore germination, in that the first division gave rise to a primary rhizoid. Thus, prothallial protoplasts
are comparable to germinating spores as suitable models for studies of developmental polarity in single cells. As in germinating
spores, the polarity of development in regenerating protoplasts is influenced by the vectors of gravity and unilateral light.
However, the relative influence of light in fixing this polarity is greater in regenerating protoplasts, while in germinating
spores, the influence of gravity is greater.
Received: 26 September 1997 / Revision received: 17 January 1998 / Accepted: 7 February 1998 |
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Keywords: | Protoplasts Fern gametophytes Prothallial protoplasts Gravity Light gradients |
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