Apoptosis and reduced influenza A virus specific CD8+ T cells in aging mice |
| |
Authors: | Zhang Y Wang Y Gilmore X Xu K Chen M Tebebi P Mbawuike I N |
| |
Institution: | Influenza Research Center, Respiratory Pathogens Research Unit, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, TX 77030, USA. |
| |
Abstract: | Some studies have reported increased apoptosis in CD8(+) T cells from aged mice. We previously demonstrated diminished virus-specific CD8(+) cytotoxic T lymphocyte (CTL) activity in aged mice in comparison to young mice. The present study investigated the role of apoptosis in age-related influenza virus-specific CD8(+) CTL deficiency. Splenocytes from influenza-primed aged and young mice were stimulated in vitro with virus. The CD8(+) T cell/total lymphocyte ratios correlated with CTL activity and were significantly decreased and increased in aged and young mice, respectively. Fas, FasL, TNF-alpha and TNFR-p55 expression, measured by flow cytometry, ELISA and/or RT-PCR, were significantly elevated in aged mice. Apoptotic CD8(+) T cells (Annexin V binding) were also elevated in aged mice. IL-12 treatment increased CD8(+) CTL activity and IFN-gamma production but did not affect apoptosis. Thus, apoptosis may contribute to reduced influenza virus-specific CD8(+) T cell frequency, CTL deficiency and increased influenza disease in aging. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|