生物素标记贾第虫全基因组DNA探针的制备及其特异性敏感性测定

用生物素标记的贾第虫全基因组ＤＮＡ探针，在斑点杂交试验中显示高度的敏感性和特异性。用它可检出１０ｎｇ贾第虫ＤＡ，１０＾３个贾第虫滋养体或包囊，且不与阴道毛滴虫、溶组织内阿米巴、弓形虫和ＢＡＢＬ／ｃ小鼠肝细胞ＤＮＡ，以及贾第虫患者粪便上清液发生交叉反应。本探针可用于贾第虫病病原体检测和虫株鉴定研究。

THE PREPARATION OF A TOTAL GENOMIC DNA PROBE OF Giardia lamblia LABELLED WITH BIOTIN AND THE DETECTION OF IT'S SPECIALITY AND SUSCEPTIVITY

An total genomic DNA probe of Giardia lamblia labelled with biotin was prepared.High sensitivity and speciality of the probe were demonstrated in the dot blot test.A hybridisation signal with 10ng of G.lamblia DNA,and 103 trophozoites or cysts was obtained by using this probe.There was no cross-reation with the closely related DNA from Trichomonas vaginalis,Entoamoeba histolytica and Toxoplasma gondii.Also,no crosshybridisations were found with the liver cells DNA from BABL/c mice as well as the fecal supernatant of G.lamblia infected subject.This probe can be used to detect G.lamblia and identify different isolates of this protozoa.