A fern spore storage protein is genetically similar to the 1.7 S seed storage protein ofBrassica napus |
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Authors: | Thomas S. Templeman Diana B. Stein Augustus E. DeMaggio |
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Affiliation: | Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755. |
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Abstract: | The ostrich fern, Matteuccia struthiopteris L., contains two globulin spore storage proteins of 2.2 S and 11.3 S, with physical characteristics similar to those of seed storage proteins of Brassica napus (rapeseed) and Raphanus sativus (radish). By the use of a cloned cDNA that encodes the 1.7 S B. napus storage protein (napin), gene sequences that hybridized with napin were detected in fern nuclear DNA, and a 900-nucleotide homologous mRNA was detected in developing spores. In vitro translation of this fern mRNA produced a 22-kD polypeptide comparable in size to the 21-kD precursor polypeptide identified in Brassica. No hybridizations were observed between the Brassica 12 S clone and either fern DNA or developing spore mRNA. |
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