Long-term ex vivo and in vivo monitoring of tumor progression by using dual luciferases |
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Authors: | Naoki Morita Sanae Haga Yoshihiro Ohmiya Michitaka Ozaki |
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Institution: | 1. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Hokkaido 062-8517, Japan;2. Laboratory of Molecular and Functional Bio-Imaging, Faculty of Health Sciences, Hokkaido University, Sapporo, Hokkaido 060-0812, Japan;3. DAILAB, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan |
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Abstract: | We propose a new concept of tumor progression monitoring using dual luciferases in living animals to reduce stress for small animals and the cost of luciferin. The secreted Cypridina luciferase (CLuc) was used as an ex vivo indicator to continuously monitor tumor progression. On the other hand, the non-secreted firefly luciferase was used as an in vivo indicator to analyze the spatial distribution of the tumor at suitable time points indicated by CLuc. Thus, the new monitoring systems that use dual luciferases are available, allowing long-term bioluminescence imaging under minimal stress for the experimental animals. |
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Keywords: | Tumor progression Ex  vivo real-time monitoring In  vivo real-time monitoring Dual reporter Cypridina luciferase (CLuc) |
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