Fatty acid analysis of triacylglycerols: Preparation of fatty acid methyl esters for gas chromatography |
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| Authors: | Ken'ichi Ichihara Chihiro Kohsaka Naohiro Tomari Tamami Kiyono Jun Wada Kiyoo Hirooka Yoshihiro Yamamoto |
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| Affiliation: | 1. Kyoto Integrated Science & Technology Bio-Analysis Center, Advanced Science, Technology & Management Research Institute of Kyoto (ASTEM), Kyoto 600-8813, Japan;2. Kyoto Municipal Institute of Industrial Technology and Culture, Kyoto 600-8815, Japan |
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| Abstract: | A method to prepare fatty acid methyl esters was developed for fatty acid analysis of triacylglycerols by gas chromatography (GC). Triacylglycerols were mixed with methanolic CH3ONa in hexane containing a mid-polar solvent for 10 s at room temperature. Under these conditions, trioleoylglycerol was converted to methyl oleate with an average yield of 99.3%. This procedure gave reliable and reproducible data on fatty acid compositions determined by GC. |
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| Keywords: | Fatty acid analysis Fatty acid methyl ester Triacylglycerol Gas chromatography |
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