Flow injection amperometric detection of insulin at cobalt hydroxide nanoparticles modified carbon ceramic electrode |
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| Authors: | Esmaeil Habibi Eskandar Omidinia Hassan Heidari Maryam Fazli |
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| Affiliation: | 1. Nanotechnology Research Center, Urmia University, Urmia, Iran;2. Young Researchers and Elite Club, Islamic Azad University of Karaj, Karaj, Iran;3. Enzyme Technology Laboratory, Biochemistry Department, Genetic and Metabolism Group, Pasteur Institute of Iran, Tehran, Iran;4. Department of Chemistry, Azarbaijan Shahid Madani University, Tabriz, Iran |
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| Abstract: | Cobalt hydroxide nanoparticles were prepared onto a carbon ceramic electrode (CHN|CCE) using the cyclic voltammetry (CV) technique. The modified electrode was characterized by X-ray diffraction and scanning electron microscopy. The results showed that CHN with a single-layer structure was uniformly electrodeposited on the surface of CCE. The electrocatalytic activity of the modified electrode toward the oxidation of insulin was studied by CV. CHN|CCE was also used in a homemade flow injection analysis system for insulin determination. The limit of detection (signal/noise [S/N] = 3) and sensitivity were found to be 0.11 nM and 11.8 nA/nM, respectively. Moreover, the sensor was used for detection of insulin in human serum samples. This sensor showed attractive properties such as high stability, reproducibility, and high selectivity. |
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| Keywords: | Flow injection analysis Insulin sensor Cobalt hydroxide Carbon ceramic Electrocatalysis |
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