The use of agar for gel electrophoresis of DNA |
| |
Authors: | C N Bush D S Holmes |
| |
Affiliation: | Department of Biological Sciences, State University of New York at Albany, 1400 Washington Avenue, Albany, New York 12222 USA |
| |
Abstract: | Agar can be used instead of agarose for electrophoresis of DNA. DNA restriction fragments migrate in proportion to the log of their molecular weights in the ranges studied. Bands of both restriction fragments and discrete small low molecular weight DNAs such as plasmids are sharp and clearly visible. The DNA can be Southern blotted with very low nonspecific background binding of radioactivity. Fragments can be removed from the gel and can be further restricted and ligated. Plasmid DNA retains its capacity to transform host bacterial cells. Agar is about the cost of electrophoresis-grade agarose. |
| |
Keywords: | To whom correspondence should be addressed. |
本文献已被 ScienceDirect 等数据库收录! |