Development of two PCR assays for the identification of mycoplasmas causing contagious agalactia |
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Authors: | Laurence Dedieu Viviane Mady Pierre-Charles Lefevre |
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Institution: | Laboratoire "PATHOTROP", CIRAD-EMVT, 10 rue Pierre Curie, 94704 Maisons-Alfort, France |
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Abstract: | Abstract A new detection test for the mycoplasmas causing contagious agalactia, Mycoplasma agalactiae , M. capricolum subsp. capricolum and M. mycoides subsp. mycoldes L.C., was developed. It was based on two polymerase chain reaction assays: the Ma-PCR for the detection of M. agalactiae and the MYC-PCR for the 'mycoides cluster' thus including M. capricolum subsp. capricolum and M. mycoides subsp. mycoides L.C. An M. agalactiae strain was identified by a 933-bp Ma-PCR product and no amplification with the MYC-PCR. In contrast, a 460-bp MYC-PCR product and a negative or a 350-bp Ma-PCR product characterized a 'mycoides cluster' strain. M. capricolum subsp. capricolum and M. mycoides subsp. mycoides L.C. were identified by their species-specific Asel pattern of the 460-bp MYC-PCR product. |
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Keywords: | Contagious agalactia Polymerase chain reaction Mycoplasma agalactiae Mycoplasma capricolum subsp capricolum Mycoplasma mycoides subsp mycoides LC |
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