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Characterizing the production of a wild-type and benomyl-resistant Fusarium lateritium for biocontrol of Eutypa lata on grapevine
Authors:G McMahan  W Yeh  M N Marshall  M Olsen  S Sananikone  J Y Wu  D E Block  J S VanderGheynst
Affiliation:(1) Department of Biological and Agricultural Engineering, University of California, Davis, CA 95616,;(2) Department of Chemical Engineering, University of California, Davis, CA 95616,;(3) Department of Viticulture and Enology, University of California, Davis, CA 95616, USA, US
Abstract:Benomyl-resistant (BR) and wild-type (WT) strains of Fusarium lateritium were examined for their tolerance to benomyl on potato dextrose agar (PDA) containing benomyl and control of the Eutypa lata in grapevine bioassays. The WT strain grew on PDA containing 1 μg/ml benomyl at 13, 26 and 29°C. The BR strain grew on PDA containing 10 μg/ml benomyl at 4°C, on PDA containing 100 μg/ml benomyl at 29°C, and on PDA containing 1000 μg/ml benomyl at 13 and 26°C. The BR strain was also able to colonize grapevine segments and control E. lata in the presence of 1000 μg/ml benomyl. Both strains were amenable to production via liquid fermentation and both achieved 100% control of E. lata in grapevine bioassays. Neither the duration of fermentation nor incubation temperature during grapevine bioassays influenced the efficacy of either strain against E. lata. The results suggest that application of BR F. lateritium alone or in combination with benomyl may provide good control of E. lata. Journal of Industrial Microbiology & Biotechnology (2001) 26, 151–155. Received 22 December 1999/ Accepted in revised form 20 October 2000
Keywords:: biological controls   Eutypa dieback   Eutypa lata, benomyl   Fusarium lateritium   grapevine
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