Bioinsecticidal activity of Talisia esculenta reserve protein on growth and serine digestive enzymes during larval development of Anticarsia gemmatalis |
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Authors: | Maria Lí gia R. Macedo,Maria das Graç as M. Freire,Carlos Eduardo G. Kubo,José Roberto P. Parra |
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Affiliation: | 1. Laboratório de Purificação de Proteínas e suas Funções Biológicas, Departamento de Tecnologia de Alimentos, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil;2. Laboratório de Química e Biomoléculas, Centro de Pesquisa, Institutos Superiores do CENSA, RJ, Brazil;3. Departamento de Entomologia, Fitopatologia e Zoologia Agrícola, Escola Superior de Agricultura Luiz de Queiroz (ESALQ), Universidade de São Paulo, CP 9, CEP 13418-900, Piracicaba, SP, Brazil |
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Abstract: | Plants synthesize a variety of molecules to defend themselves against an attack by insects. Talisin is a reserve protein from Talisia esculenta seeds, the first to be characterized from the family Sapindaceae. In this study, the insecticidal activity of Talisin was tested by incorporating the reserve protein into an artificial diet fed to the velvetbean caterpillar Anticarsia gemmatalis, the major pest of soybean crops in Brazil. At 1.5% (w/w) of the dietary protein, Talisin affected larval growth, pupal weight, development and mortality, adult fertility and longevity, and produced malformations in pupae and adult insects. Talisin inhibited the trypsin-like activity of larval midgut homogenates. The trypsin activity in Talisin-fed larvae was sensitive to Talisin, indicating that no novel protease-resistant to Talisin was induced in Talisin-fed larvae. Affinity chromatography showed that Talisin bound to midgut proteinases of the insect A. gemmatalis, but was resistant to enzymatic digestion by these larval proteinases. The transformation of genes coding for this reserve protein could be useful for developing insect resistant crops. |
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Keywords: | Anticarsia gemmatalis Gut enzymes Reserve protein Talisin Toxicity Bioinsecticide |
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