Renaturation of denatured DNA in NaI gradients |
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| Authors: | R. A. Clegg P. Borst |
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| Affiliation: | 1. Section for Medical Enzymology, Laboratory of Biochemistry, University of Amsterdam, Amsterdam, The Netherlands
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| Abstract: | We show that denatured DNA from Tetrahymena mitochondria or phage T7, partially renatures during centrifugation in NaI equilibrium density gradients. This makes these gradients unsuitable for the analysis of single-stranded complementary nucleic acids, especially if their complexity and mole percent G+C are low. |
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