| 海南部分荔枝种质资源亲缘关系的SSR分析 |
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| 引用本文： | 姚庆荣,赵长增,王文泉. 海南部分荔枝种质资源亲缘关系的SSR分析[J]. 植物研究, 2009, 29(5): 628-632 |
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| 作者姓名： | 姚庆荣 赵长增 王文泉 |
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| 作者单位： | (1.甘肃行政学院，兰州730010) ;(2.甘肃农业大学农学院，兰州730070) ;(3.中国热带农业科学院热带生物技术研究所，海口571101) |
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| 摘 要： | 利用SSR标记对22份荔枝材料进行了亲缘关系分析，从32对引物中筛选出22对多态性引物用于荔枝SSR扩增，共扩增到52条带，其中多态性条带49条，多态性百分率为94.23%。多态性条带经POPGENE32软件统计分析表明，22个位点的平均有效等位基因频率（Ne）、平均基因杂合度（H）、平均Shannon遗传多样性指数（Hi′）分别为1.364 3、0.296 0、0.417 0。通过NTSYS聚类结果显示，在相似系数为0.51处，供试材料被聚为两大类，第一类包括13份材料，又可分为两个亚类，第二类包括9份材料。
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| 关 键 词： | 荔枝；亲缘关系；SSR；海南 |
Analysis of Genetic Relationship of Hainan Litchi Germplasm Resources by SSR Marker |
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| YAO Qing-Rong ZHAO Chang-Zeng WANG Wen-Quan. Analysis of Genetic Relationship of Hainan Litchi Germplasm Resources by SSR Marker[J]. Bulletin of Botanical Research, 2009, 29(5): 628-632 |
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| Authors: | YAO Qing-Rong ZHAO Chang-Zeng WANG Wen-Quan |
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| Affiliation: | 1.Gansu Administration Institute;Lanzhou 730070;2.College of agronomy;Gansu Agricultural University;Lanzhou 730070;3.Institute of Tropical Bioscience and Biotechnology;CATAS;Haikou 571101 |
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| Abstract: | 22 Litchi germplasm were used as materials for analyzing their genome polymorphism by SSR markers. 22 primers selected from 32 primers were used for SSR amplification. A total of 52 bands were generated, of which 49 bands were polymorphic bands. The percentage of polymorphic band (PPB) was 94.23%. According to the analysis results of POPGENE32 software, the average effective number of alleles(Ne), the Nei’s average gene diversity (H) and the average of Shannon’s genetic diversity(Hi′) index was 1.364 3, 0.296 0 and 0.417 0, respectively. The UPGAM dendrogram gotten by NTNSYS cluster analysis implied that 22 Litchi germplasm resources could be divided into 2 groups on the basis of genetic distance 0.51. The fist group included 13 cultivars. The second group included 9 cultivars. |
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| Keywords: | Litchi genetic relationship SSR hainan province |
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