Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63 |
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Authors: | Habe Hiroshi Chung Jin-Sung Kato Hiroyuki Ayabe Yuko Kasuga Kano Yoshida Takako Nojiri Hideaki Yamane Hisakazu Omori Toshio |
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Institution: | Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. uhhabe@mail.ecc.u-tokyo.ac.jp |
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Abstract: | Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone to produce 9-fluorenone and 2'-carboxy-2,3-dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded by flnD1 and ORF16, being a member of the class III two-subunit extradiol dioxygenase. FlnE was identified as a serine hydrolase for the meta-cleavage products that yield phthalate. |
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