Involvement of the proton electrochemical gradient in genetic transformation in Escherichia coli |
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Authors: | E Santos H R Kaback |
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Institution: | Laboratory of Membrane Biochemistry Roche Institute of Molecular Biology, Nutley, New Jersey 07110 U.S.A |
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Abstract: | Plasmid pIY2 DNA which encodes for ampicillin-resistance was used to study the energetics of Ca++-induced transformation in Escherichia coli. When cells are exposed to DNA in the presence of carbonylcyanide-m-chlorophenylhydrazone or 2,4-dinitrophenol, two protonophores that collapse the proton electrochemical gradient across the cell membrane (), transformation to ampicillin-resistance is drastically reduced with little or no effect on viability. Furthermore, when the components of are altered by varying ambient pH or by performing transformation in the presence of valinomycin or nigericin, the efficiency of transformation is directly correlated with the magnitude of the membrane potential and changes in the pH gradient have no significant effect. It is concluded that , more specifically the membrane potential, plays a critical role in Ca++-induced transformation. |
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