Electroporation of binary Ti plasmid vector into Agrobacterium tumefaciens and Agrobacterium rhizogenes |
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| Authors: | R. Nagel A. Elliott A. Masel R.G. Birch J.M. Manners |
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| Affiliation: | Department of Botany, The University of Queensland, St. Lucia, Australia;CSIRO-UQ Plant Pathology Unit, The University of Queensland, St. Lucia, Australia |
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| Abstract: | Abstract Efficient transformation of strains of Agrobacterium tumefaciens and Agrobacterium rhizogenes by electroporation with binary Ti plasmid vector is reported. This procedure yields rates of transformation of 106-103 per μg DNA, which is several orders of magnitude greater than previously published procedures for this genus, the efficiency of transformation varies with the bacterial strain used. This procedure will be useful for the construction of plant DNA libraries directly in Agrobacterium . |
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| Keywords: | Agrobacterium Electroporation Transformation Plant gene isolation Ti plasmid vectors |
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