Association of a CONSTANS-LIKE gene to flowering and height in autotetraploid alfalfa |
| |
Authors: | Doris Herrmann Philippe Barre Sylvain Santoni Bernadette Julier |
| |
Institution: | 1. INRA, UR 4, Unité de Recherche Pluridisciplinaire Prairies et Plantes Fourragères, BP 6, 86600, Lusignan, France 3. EPFL, Coopération Indo-Suisse, CM 2 305, Station 10, 1015, Lausanne, Switzerland 2. INRA, UMR Diversité et Adaptation des Plantes Cultivées, 34060, Montpellier, France
|
| |
Abstract: | In alfalfa (Medicago sativa), an autotetraploid forage legume, stem length is a major component of forage yield, quality and competing ability. In this
species, flowering date is not a breeding criterion. Association mapping based on a candidate gene approach has given good
results in plants, including autotetraploid species for which genetic analyses are complex. The role of a CONSTANS-LIKE gene,
identified as a candidate for stem elongation and flowering date in the model legume M. truncatula, was tested for association with the same traits in alfalfa. Four hundred genotypes from ten cultivars were evaluated for
stem height and flowering date in two locations during 4 years. They were genotyped with simple sequence repeat markers and
a low structuration was noticed. Primers were designed to amplify and sequence two regions of the alfalfa gene homologous
to CONSTANS-LIKE. Single nucleotide polymorphisms (SNPs) were detected and their allelic dose in each genotype was scored.
Linkage disequilibrium within CONSTANS-LIKE rapidly decreased as expected. Eight SNPs with a frequency above 10% were detected
over 1,010 bp (one SNP every 126 bp on average) in the 400 genotypes. This number was lower than observed in a neutral gene
(a SNP every 31 bp on average). Highly significant associations of three SNPs to flowering date and stem height were identified.
Each SNP explained up to 4.2% of the genetic variance. Thus, as in the model species, the CONSTANS-LIKE gene was shown to
be involved in flowering date and stem height in alfalfa. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|