A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA |
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Authors: | Jackson David Cadman Andrew Zurcher Thomas Barclay Wendy S |
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Institution: | University of Reading, Whiteknights, Reading RG6 6AJ. GlaxoSmithKline Medicine Research Centre, Stevenage SG1 2NY, United Kingdom. |
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Abstract: | The recovery of recombinant influenza A virus entirely from cDNA was recently described (9, 19). We adapted the technique for engineering influenza B virus and generated a mutant bearing an amino acid change E116G in the viral neuraminidase which was resistant in vitro to the neuraminidase inhibitor zanamivir. The method also facilitates rapid isolation of single-gene reassortants suitable as vaccine seeds and will aid further investigations of unique features of influenza B virus. |
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