H-2 histocompatibility antigens of subcellular membranes of mouse liver |
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Authors: | D. James Morr , V. Schirrmacher, Peter Robinson, Kristine Hess,Werner W. Franke |
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Affiliation: | 1. Departments of Medicinal Chemistry and Biological Sciences, Purdue University, West Lafayette, IN 47907, USA;2. Institutes of Immunology and of Experimental Pathology, German Cancer Research Center, D-69 Heidelberg, Germany |
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Abstract: | Purified fractions of plasma membrane, Golgi apparatus, rough endoplasmic reticulum vesicles, nuclear envelope, and mitochondria were isolated from mouse liver and the distribution of H-2 histocompatibility antigens determined by indirect radioimmunoassay before and after membrane disruptive treatments. Fractions enriched in plasma membrane (surface membrane) revealed H-2 antigens in highest concentration; disruptive treatments were not necessary to reveal H-2 antigens with surface membranes. In contrast, internal membranes did not possess H-2 antigens which were accessible to antibody. Golgi apparatus fractions or some component of these fractions (e.g. secretory vesicles) possessed the antigens but in a latent form where accessibility was provided by simple rupture of the membrane vesicles. With endoplasmic reticulum, detergent solubilization of the membranes was required before H-2 antigen could be detected. Nuclear envelope preparations contained little or no demonstrable H-2 activity. These results were confirmed by several techniques including immunoprecipitation of labelled solubilized membrane components with anti-H-2 serum and subsequent analysis in SDS-PAGE. |
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