Granuloma formation in vitro requires B-1 cells and is modulated by Paracoccidioides brasiliensis gp43 antigen |
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Authors: | Vigna Ana Flávia Almeida Sandro Rogério Xander Patricia Freymüller Edna Mariano Mario Lopes José Daniel |
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Institution: | 1. Department of Applied Sciences, Northumbria University, Ellison Building, Newcastle Upon Tyne NE1 8ST, UK;2. Department of Geography, Northumbria University, Ellison Building, Newcastle Upon Tyne NE1 8ST, UK.;1. Department of Immunology and Transfusion Medicine, Faculty of Medicine, University of Oslo and Oslo University Hospital Rikshospitalet, Oslo, Norway;2. Department of Neurology, Akershus University Hospital, Lørenskog, Norway;3. Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway;4. Proteomics Core Facility, Oslo University Hospital Rikshospitalet, NO-0372 Oslo, Norway |
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Abstract: | The mechanisms that determine granuloma formation and the significance of this type of inflammatory response in the pathogenesis of fungal diseases such as paracoccidioidomycosis are far from fully understood. We developed a granuloma model in vitro using beads to evaluate the role of isolated mouse peritoneal macrophages and B-1 cells. We also investigated granuloma formation in the presence of gp43, the main antigenic component of Paracoccidioides brasiliensis, which is secreted exocellularly. To determine whether B-1 cells, macrophages, or both, participate in granuloma formation, peritoneal cells from Xid mice, which lack B-1 cells, were used. Granuloma-like structures were not formed with Xid peritoneal cells or with cells from wild type mice that had their peritoneal and pleural cavities irradiated before the cultures were established. Granulomas were observed either when total adherent peritoneal cells or when isolated B-1 cells were added to macrophage cultures. The data strongly suggest that an interaction of B-1 cells and macrophages plays an important role in granuloma-like formation in this experimental model and that the presence of gp43 strongly stimulates this response. |
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