| 醛固酮对大鼠主动脉bax基因表达的影响 |
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| 引用本文： | 闫永吉,王超,吴准,李俊,马鑫,李宏召,王保军,史涛坪,龚道静,倪栋,张旭. 醛固酮对大鼠主动脉bax基因表达的影响[J]. 国外医学:分子生物学分册, 2010, 0(1): 1-5 |
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| 作者姓名： | 闫永吉 王超 吴准 李俊 马鑫 李宏召 王保军 史涛坪 龚道静 倪栋 张旭 |
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| 作者单位： | [1]华中科技大学同济医学院附属同济医院泌尿外科,武汉市430030 [2]山东省济宁市第一人民医院泌尿外科,山东省济宁市272000 [3]中国人民解放军总医院泌尿外科,北京市100853 |
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| 基金项目： | 国家杰出青年科学基金（No.30725040） |
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| 摘 要： | 目的研究醛固酮对大鼠主动脉bax基因表达的影响。方法32只SD大鼠随机分为空白对照组、腺瘤组、腺瘤＋依普利酮组和腺瘤＋肼苯哒嗪组。在每只大鼠皮下埋植的微量渗透泵内注入空白溶剂或醛固酮。8周后通过免疫组化、RT—PCR和Western印迹检测主动脉bax基因的表达。结果与对照组相比,腺瘤组大鼠主动脉bax mRNA和蛋白表达都显著上调（P〈0．05）;依普利酮能够抑制醛固酮对bax基因的诱导作用（P〈0．05）;而肼苯哒嗪虽然可以使大鼠收缩压下降,但不能阻止醛固酮对bax基因的作用。结论醛固酮通过诱导bax基因表达,调节血管平滑肌细胞凋亡和干预细胞周期进程,可能是其导致血管重构的机制之一。
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| 关 键 词： | 醛固酮 bax基因 依普利酮 肼苯哒嗪 基因表达与调控 |
Effect of Aldosterone on bax Gene Expression in Rat Aorta |
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| YAN Yongji,WANG Chao,WU Zhun,LI Jun,MA Xin,LI Hongzhao,WANG Baojun,SHI Taoping,GONG Daojing,NI Dong,ZHANG Xu. Effect of Aldosterone on bax Gene Expression in Rat Aorta[J]. , 2010, 0(1): 1-5 |
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| Authors: | YAN Yongji WANG Chao WU Zhun LI Jun MA Xin LI Hongzhao WANG Baojun SHI Taoping GONG Daojing NI Dong ZHANG Xu |
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| Affiliation: | 1Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science ＆ Technology, Wuhan, 430030, China 2Department of Urology, First People＇s Hospital of Jining City, Jining , Shandong , 272000, China 3Department of Urology, Chinese People＇s Liberation Army General Hospital, Military Postgraduate Medical College, Beijing, 100853, China) |
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| Abstract: | Objective To study the effect of aldosterone on expression of bax gene in rat aorta. Methods 32 Sprague-Dawley rats bearing an osmotic mini-pump that infuses vehicle or aldosterone （1 μg/h ）, were randomly divided into 4 groups： control; adenoma; adenoma plus eplerenone （100 mg/kg/day ） and adenoma plus hydralazine （25 mg/kg/day） . After 8 weeks, the expression of bax gene in the aorta was examined at both rnRNA and protein levels by immunohistochemistry, RT-PCR and Western blot. Results Compared with controls, the expression of bax mRNA and protein in the aorta was significantly upregulated in adenoma group （ P 〈 0. 05 ） . This upregulation was inhibited by eplerenone but not by hydralazine. Conclusion The upregulated bax gene by aldosterone via mineralocorticoid receptor independently of blood pressure, may be involved in aldosterone-induced vascular remodeling. |
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| Keywords: | aldosterone bax gene eplerenone hydralazine gene expression and regulation |
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