酵母双杂交系统在膜蛋白相互作用研究中的应用

膜相关蛋白约占细胞总蛋白质中的1/3，它们大都参与了细胞的诸多生理、病理过程和药物反应机理。研究膜蛋白的相互作用对于揭示细胞的生命活动规律及寻找药物作用靶标都有重要的意义。由于膜蛋白本身的特性及其难以进入核内等原因，经典的酵母双杂交技术并不适用于检测膜蛋白间的相互作用。针对在活细胞中研究膜蛋白相互作用的需要，近年来国际上先后发展了一系列用于膜蛋白相互作用研究的酵母双杂交新系统，并取得了许多重要发现。

Advanced Application of Yeast Two-Hybrid System in Studying Membrane Protein Interaction

Membrane associated proteins account for approximately a third of total proteins in a live cell. Most of the membrane proteins are directly involved in physiological and pathological processes and pharmacological response mechanism. Identification of interactions between membrane proteins can help to unveil regulatory mechanism of cellular life activity and discover drug targets. Conventional yeast two-hybrid system has achieved important results and abroad application in research of nuclear protein-protein interaction, However, it is different to identify interactions between membrane proteins due to its hydrophobic nature and incapability of nuclear translocation. In order to meet the need of studying membrane protein interaction in live cells, some novel yeast two-hybrid systems have been successively evolved and established in the recent years, leading to exciting im portant findings. This review summarizes the basic principles and advanced applications of reverse Ras recruitment system, guanine-nucleotide binding protein fusion assay and split-ubiquitin assay systems.