| 血红素加氧酶-1对肝癌细胞细胞周期调控因子的影响 |
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| 引用本文： | 高涵,邹朝霞,刘文杰,张宏宇,高旭. 血红素加氧酶-1对肝癌细胞细胞周期调控因子的影响[J]. 国外医学:分子生物学分册, 2010, 0(2): 99-103 |
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| 作者姓名： | 高涵 邹朝霞 刘文杰 张宏宇 高旭 |
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| 作者单位： | [1]哈尔滨医科大学生物化学与分子生物学教研室,哈尔滨市150081 [2]齐齐哈尔医学院生物化学与分子生物学教研室,黑龙江省齐齐哈尔市161006 |
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| 基金项目： | 黑龙江省卫生厅基金（No.2009-211）,黑龙江省留学归国基金（No.LC06C21）,黑龙江省教育厅科技计划项目（No.115hz24） |
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| 摘 要： | 目的观察血红素加氧酶-1（heme oxygenase 1，HO-1）对人肝癌细胞HepG2细胞周期调控因子的影响。方法构建含有野生型和突变型HO-1基因的重组载体pcDNA3．1（＋）-wtHO-1和pcDNA3．1（＋）-mHO-1G143H。利用脂质体介导的方法将构建好的重组载体转染肝癌细胞系HepG2，以空载体转染作为对照组。通过G418筛选建立稳定表达野生型和突变型HO-1的HepG2肝癌细胞系。经半定量RT—PCR、Western印迹检测转染细胞系中HO-1 mRNA和蛋白的表达水平。在HO-1表达改变的稳转细胞系中，利用Western印迹检测转染细胞系中P21、P27蛋白表达水平。结果成功实现了野生型和突变型HO-1在HepG2细胞中的过表达；野生型和突变型HO-1过表达均能诱导抑癌基因p21和p27的表达。结论HO．1过表达诱导抑癌基因p21和p27的表达与血红素分解产物无关。HO-1可能通过其它机制调节p21和p27的表达。
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| 关 键 词： | 血红素加氧酶－1 肝癌 细胞周期 细胞周期调控因子 |
Effect of Heme Oxygenase-1 on Cell Cycle Regulatory Factors in Liver Cancer Cells |
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| GAO Han,ZOU Chaoxia,LIU Wenjie,ZHANG Hongyu,GAO Xu. Effect of Heme Oxygenase-1 on Cell Cycle Regulatory Factors in Liver Cancer Cells[J]. , 2010, 0(2): 99-103 |
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| Authors: | GAO Han ZOU Chaoxia LIU Wenjie ZHANG Hongyu GAO Xu |
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| Affiliation: | 1Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang, 150081, China 2Department of Biochemistry and Molecular Biology, Qiqihar Medical College, Qiqihar, Heilongfiang, 161006, China) |
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| Abstract: | Objective To elucidate the effect of heme oxygenase-1 on cell cycle regulator in HepG2 cell line. Methods To construct eukaryotic expression vectors expressing wild-type HO-1 and G143H mutant HO-1, named pcDNA3.1（ ＋ ） -wtHO-1 and pcDNA3.1（ ＋ ） - mHO-1G143H. HepG2 cells were transfected with wtHO-1 and mHO-1 using lipofectamine 2000. Stable transfected cells were selected by G418. Expression of HO-1 mRNA and protein were detected using RT-PCR and Western blot respectively. In addition, expression of p21 and p27, the major eyclin-dependent kinase （Cdk） inhibitors in the two cell lines were examined. Results HepG2 cell lines with wild type and mutant HO-1 overexpression were established successfully. Overexpression of both wild type and mutant HO-1 enhanced the expression of tumor suppressor P21 and P27. Conclusion HO- 1 overexpression upregulated the level of P21 and P27. This regulation is not dependent of hHO-1 catalytic activity, but probably other mechanisms. |
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| Keywords: | heine oxygenase-1 hepatoma cell cycle cell cycle regulators |
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