脂多糖对人支气管上皮细胞STAT1、STAT3、STAT4、STAT6表达的影响

目的观察脂多糖对人支气管上皮细胞16HBESTAT1、STAT3、STAT4、STAT6表达的影响。方法采用普通RT—PCR检测16HBE细胞STAT1、STAT3、STAT4、STAT6的mRNA表达；Western印迹检测16HBE细胞STAT1、STAT4、STAT6的蛋白表达。分别采用不同浓度的脂多糖在不同的时间点处理16HBE细胞，采用Real—timePCR的方法检测16HBE细胞STAT1、STAT3、STAT4、STAT6的mRNA表达。结果1μg／m1的LPS处理16HBE细胞1h组、0．25μg／m1的LPS处理16HBE细胞4h组、1μg／ml的LPS处理16HBE细胞4h组STAT1、STAT4的mRNA表达较正常对照组显著增高（P〈0．01）；0．25μg／ml的LPS处理16HBE细胞2h组、1μg／ml的LPS处理16HBE细胞2h组、10μg／ml的LPS处理16HBE细胞2h组STAT1、STAT4的mRNA表达较正常对照组有所增高（P〈0．05）；1μg/ml的LPS处理16HBE细胞1h组STAT6的mRNA表达较正常对照组显著增高（P〈0．01）。所有LPS处理16HBE细胞组STAT3的mRNA表达均较正常对照组减低。结论人支气管上皮细胞表达STAT1、STAT3、STAT4、STAT6的mRNA和STAT1、STAT4、STAT6的蛋白，一定剂量的脂多糖在某些时间点分别刺激了人支气管上皮细胞STAT1、sTAT4、STAT6的mRNA表达。

Effect of Lipopolysaccharide on STATI, STAT3, STAT4 and STAT6 Expression in Human Bronchial Epithelial Cells

Objective To elucidate the effect of Lipopolysaccharide （LPS） on STAT1, STAT3, STAT4 and STAT6 expression in human bronchial epithelial cell line 16HBE. Methods STAT1, STAT3, STAT4 and STAT6 mRNA expression in 16HBE were detected by using RT-PCR; STAT1, STAT4 and STAT6 protein expression in 16HBE were detected by using Western Blot. We stimulated 16HBE with different concentration LPS at some interval, and detected STAT1, STAT3, STAT4 and STAT6 mRNA expression in 16HBE by using Real-time PCR. Results The mRNA expression of STAT1, STAT4 in 16HBE treated by 1μg/ml LPS at 1 h, 0. 25μxg/ml LPS at 4 h, 1μg/ml LPS at 4 h were higher significantly than normal controls （P 〈0. 01 ） ; the mRNA expression of STAT1, STAT4 in 16HBE treated by 0. 25μg/ml LPS at 2 h, 1μg/ml LPS at 2 h, 10μg/ ml LPS at 2 h were higher than normal controls （ P 〈 0. 05 ） . The mRNA expression of STAT6 in 16HBE treated by 1μg/ml LPS at 1 h was higher significantly than normal controls （P 〈 0. 01 ） . And the mRNA expression of STAT3 in 16HBE treated by different concentration LPS at various intervals were lower than corresponding normal controls. Conclusion The mRNA expression of STAT1, STAT3, STAT4 and STAT6 were detected in 16HBE ; the protein expression of STAT1, STAT4 and STAT6 were detected in 16HBE. The mRNA expression of STAT1, STAT4 and STAT6 were up-regulated in 16HBE by some concentration LPS at some interval.