Intrinsic bent DNA colocalizes with the sequence involved in the Nd-sD mutation in the Bombyx mori fibroin light chain gene |
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Authors: | Barbosa Joice Felipes Bravo Juliana Pereira Takeda Karen Izumi Zanatta Daniela Bertolini Silva Jose Luis da Conceicao Balani Valerio Americo Fiorini Adriana Fernandez Maria Aparecida |
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Affiliation: | Departamento de Biologia Celular e Genetica, Universidade Estadual de Maringa, Maringa, Parana, Brazil. aparecidafernandez@gmail.com |
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Abstract: | Multiple sequence alignments of the Bombyx mori fibroin light chain gene (fib-L) from hybrids and from Chinese and Japanese strains demonstrated that 51.6% of the fib-L third intron is conserved. One of these conserved segments, 41 bp long, contains the sequence CGTTATTATACATATT, which is duplicated in the B. mori Nd-s(D) mutant. In the present work, electrophoretic mobility assays and computational analyses revealed a major peak of intrinsic bent DNA within the segment that undergoes breakage in the previously-described Nd-s(D) mutation. This result suggested that this intrinsically-curved region might mediate DNA cleavage and enhance recombination events in the third intron of the Bombyx mori fib-L gene. |
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