Sequence analysis of a chloroplast intergenic spacer for phylogenetic estimates in Allium section Cepa and a PCR-based polymorphism detecting mixtures of male-fertile and male-sterile cytoplasmic onion |
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Authors: | J W Lilly M J Havey |
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Institution: | (1) Vegetable Crops Unit, United States Department of Agriculture, Agricultural Research Service, Department of Horticulture, 1575 Linden Drive, Univ. of Wisconsin, Madison, WI 53706, USA Fax: +1 608–262–4743 e-mail: mjhavey@facstaff.wisc.edu, US |
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Abstract: | Restriction-enzyme analysis of the chloroplast (cp) DNA yielded maternal phylogenies supporting a close phylogenetic relationship
among normal (N) male-fertile and male-sterile (S) cytoplasmic bulb onion (Allium cepa), Allium altaicum, Allium fistulosum, Allium galanthum, Allium roylei, and Allium vavilovii. The S cytoplasm of onion is most likely an alien cytoplasm introduced in antiquity into onion populations. We previously
showed that size differences in an intergenic spacer in the cp DNA distinguish N and S cytoplasms of onion. We cloned and
sequenced this intergenic spacer from the N and S cytoplasms of onion, A. altaicum, A. fistulosum, A. galanthum, Allium pskemense, Allium oschaninii, A. roylei, and Allium ampeloprasm (outgroup) to identify the nature of previously described RFLPs and to develop a PCR-based marker revealing N-cytoplasmic
contamination of S-cytoplasmic hybrid seed lots. Phylogenies based on restriction-enzyme analysis of the entire cp DNA were
similar, but not identical, to those based on sequence divergence in this intergenic region.
Received: 29 November 1999 / Accepted: 28 April 2000 |
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Keywords: | Cytoplasmic male sterility Polymerase chain reaction Allium cepa |
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