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斜纹夜蛾两个天蚕素D基因的克隆及序列分析
引用本文:陈维春,宋杰,庞义. 斜纹夜蛾两个天蚕素D基因的克隆及序列分析[J]. 昆虫学报, 2007, 50(7): 745-749
作者姓名:陈维春  宋杰  庞义
作者单位:(中山大学有害生物控制与资源利用国家重点实验室,昆虫学研究所,广州 510275)
摘    要:天蚕素是昆虫抵御病菌入侵的一类抗菌肽家族。根据斜纹夜蛾Spodoptera litura天蚕素B基因设计特异引物,通过PCR扩增得到2个新的天蚕素基因部分序列,分别命名为cecD1cecD2(GenBank登录号分别为EF555567和EF555568)。2个基因编码同一个天蚕素D蛋白,该蛋白的成熟肽与天蚕素B存在2个氨基酸残基差异。序列分析发现cecD1cecD2中分别包含568 bp和377 bp的内含子序列,它们有相同的5′和3′拼接位点,A+T含量分别为59.7%和69.8%,符合大多数真核生物内含子高A+T含量的特征。

关 键 词:斜纹夜蛾  抗菌肽  天蚕素  内含子  基因结构  
文章编号:0454-6296(2007)07-0745-05
收稿时间:2007-02-15
修稿时间:2007-04-27

Cloning and sequence analysis of two cecropin D genes from the common cutworm, Spodoptera litura ( Lepidoptera: Noctuidae)
CHEN Wei-Chun,SONG Jie,PANG Yi. Cloning and sequence analysis of two cecropin D genes from the common cutworm, Spodoptera litura ( Lepidoptera: Noctuidae)[J]. Acta Entomologica Sinica, 2007, 50(7): 745-749
Authors:CHEN Wei-Chun  SONG Jie  PANG Yi
Affiliation:(State Key Laboratory for Biocontrol, Institute of Entomology, Sun Yat-Sen University, Guangzhou 510275, China)
Abstract:Cecropins are a family of antibacterial peptides synthesized in insects as a response to a bacterial infection. Two primers were designed according to cecropin B gene from Spodoptera litura and were used to PCR. As the result, two new cecropin genes, named cecD1 and cecD, were obtained, which encoded identical preprocecropin D. There was 2 amino acid residues difference between the mature peptide of preprocecropin D and that of cecropin B from S. litura. Two introns, 568 bp and 377 bp long, were found in cecD1 and cecD2, respectively, both having the same 5′and 3′splicing sites. The A+T content was 59.7% in cecD1 intron and 69.8% in cecD2 intron, conforming to the high A+T content characteristics of most eukaryotic introns.
Keywords:Spodoptera litura  antibacterial peptide  cecropin  intron  gene structure
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