新城疫病毒分离株HN和P基因分子进化及其相关研究

为探讨新城疫病毒(Newcastle disease virus,NDV)血凝素-神经氨酸酶(HN)和磷蛋白(P)基因遗传特性以及相互关系,将1997～2005年间国内分离到12株NDV毒株,分别进行HN和P基因克隆测序,结合15个已发表的国内外不同时期的NDV毒株HN和P基因,计算所有毒株HN和P基因的不同核苷酸和氨基酸片段进化距离,利用统计软件进行了不同片段间进化距离的方差分析,HN或P基因核苷酸进化距离与毒株分离时间、HN或P基因片段与其全长间以及HN和P基因全长间的相关分析.统计分析显示:NDV HN或P基因不同核苷酸和氨基酸序列片段变异程度不一样;不同毒株间HN或P基因片段与其全长间以及HN和P基因全长间无论是核苷酸还是氨基酸遗传变异高度相关.以上说明,NDV HN和P基因虽以不同的方式进化,但是HN和P基因遗传变异的趋势是相同的.HN和P基因的变异与分离时间有一定的联系.

Molecular Evolution and Correlation of HN and P Gene Among the Field Newcastle Disease Viruses

The goal of this study is to research the genetic characteristics and relationship between HN and P genes of NDV.The nucleotide sequence and deduced amino acid sequence were analyzed for the Hemagglutinin-neuramindase(HN)and Phosphoprotein(P)gene of twelve field isolates of Newcastle disease virus(NDV)during 1997-2005 in China.The HN and P gene sequences of fifteen NDV reference strains from GenBank were also used in this study.The molecular evolution distance of nucleotides and amino acids were calculated by MEGA 4.0 software,and analysis of variance and correlations were analyzed by SPSS11.0 software among different length sequences of the HN gene or P gene.The nucleotide and amino acids correlation of HN and P gene were analyzed respectively.The correlation of evolution distance and isolation year were also calculated.The results indicated that there were difference and good correlation of nucleotide and amino acid among different length sequences of the HN gene or P gene.These results revealed that the HN and P gene of NDV have the different response to selective pressure to adopt to landscape and closely relationship on heredity mutations.Nucleotide variations of HN and P gene have relationship with isolation year of strains.