Metabolism and Biosynthesis of Cytokinins in Tobacco Crown Gall Cells |
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Authors: | Nakajima Hiromitsu; Yokota Takao; Takahashi Nobutaka |
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Institution: | Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo 113, Japan
1Department of Agricultural Chemistry Tottori University, Koyama, Tottori 680, Japan. |
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Abstract: | The metabolism of ribosylzeatin (RZ) was studied using tobaccocrown gall cells which produce RZ as one of the major endogenouscytokinins. When 8-14C]RZ was fed to the cells, it was convertedinto its phosphate (which was rigorously determined to be the5'-monophosphate), RZ-O-glucoside, inosine (or its phosphate),adenosine and adenosine-O-glucoside. When 8-14C]N6-( 2-isopentenyl)adenosine(i6Ado), a probable precursor of RZ, was fed to the cells, itwas converted into (i6Ado)-O-glucoside, inosine (or its phosphate),adenosine, adenosine-O-glucoside and adenosine phosphate, butno incorporation of radioactivity into RZ was observed. Thepresent study led to the following conclusions: i) i6Ado isnot a precursor of RZ in the cells, ii) both deaminase and cytokininoxidase are involved in the catabolism of cytokinin, and iii)the metabolism of RZ is quite different from that of i6Ado. (Received December 24, 1985; Accepted April 1, 1986) |
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