牛磺酸及微量营养素对大鼠视网膜NOS表达及cGMP含量影响的研究

目的：探讨大鼠补充一定剂量牛磺酸及微量营养素后，能否通过影响视感受器或视中枢ＮＯ合成酶（ＮＯＳ）表达及第二信使（ｃＧＭＰ）合成，影响视觉信号传导。方法：Ｗｉｓｔａｒ大鼠随机分为三组，即对照组（正常饲料组）、实验１组（５倍需要量组）和实验２组（１０倍需要量组），喂养３周后，每组动物再随机分为光照组和暗适应组（平均照度为３．０３ＬＸ），以正常饲料喂养７２ｈ，大鼠活杀取样，以放射免疫方法分析ｃＧＭＰ含量

INFLUENCES OF TAURINE AND MICRONUTRIENTS ON NITRIC OXIDE SYNTHASE EXPRESSION AND cGMP CONTENT IN RAT RETINA

Aim: To inverstigate the influence of taurine and micronutrients on visual signal transmission. Methods: Wistar rats were divi ded into three groups, that is control group, experiment group 1 and experiment group 2, and fed for 3 weeks with normal diet, 5 times and 10 times doses of requirements of taurine, vitamin A, vitamin B, zinc and selenium, then each treatment group were divi ded into light group and dark adaptation group. After feeding another 3 days in different environments with normal diet, all animals were killed and cGMP level and NOS expression were analysed in retina and retinogenicualte.Results:The NOS expression and cGMP contents of photoreceptor cells, visual cortex and retinogeniculate were increased in dark adaptation group compared with light group. Nutritional intervention could enhance the NOS staining in dark environment, increased the cGMP contents whether light or dark condition. Conclusion: The distribution, expression and content of NO and cGMP are quite different in various light adaptation status. Taurine and micronutrient intervention may modurate the visual signal transmission or vision function mediated by the changes of NO or cGMP.