Mechanisms of damage to sperm structure in mice on the zinc-deficient diet |
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| Affiliation: | 1. Graduate School of Hebei Medical University, Shijiazhuang 050017, China;2. Hebei Key Laboratory of Reproductive Medicine, Hebei Institute of Reproductive Health Science and Technology, Shijiazhuang 050071, China;3. Chengde Medical College, Chengde 067000, China;4. School of Chemistry and Materials Science, Hebei Normal University, Shijiazhuang 050024, China;1. Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, Changsha 410078, China;2. Center of Clinical Pharmacology, the Third Xiangya Hospital, Central South University, Changsha 410013, China;3. Environmental Science and Engineering, College of Resource and Environment, Hunan Agricultural University, Changsha 410128, China;1. Department of Chemistry, Division of Science & Technology, University of Education, Lahore, Pakistan;2. Department of Applied Chemistry, Government College University, Faisalabad 38000, Pakistan;3. Department of Chemistry, Government College University, Faisalabad 38000, Pakistan;4. Division of Pulmonary Critical Care and Sleep Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA;1. School of Biopharmacy, China Pharmaceutical University, Nanjing 211198, PR China;2. School of Medicine, and Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210023, PR China;3. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, PR China;1. Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India;2. Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India;1. Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Iran;2. Department of Animal Science, faculty of Agriculture, University of Tabriz, Iran;1. Department of Occupational Health, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi 030001, China;2. Key Lab of Environmental Hazard and Health of Shanxi Province, Shanxi Medical University, Taiyuan, Shanxi 030001, China;3. Key Lab of Cellular Physiology of Education Ministry, Shanxi Medical University, Taiyuan, Shanxi 030001, China;4. Department of Anatomy, Shanxi Medical University, Taiyuan, Shanxi 030001, China |
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| Abstract: | BackgroundZinc (Zn)is an essential trace element for spermatogenesis and its deficiency causes abnormal spermatogenesis.ObjectiveThe present study was conducted to examine the mechanisms by which Zn-deficient diet impairs sperm morphology and its reversibility.Methods30 SPF grade male Kunming (KM) mice were randomly divided into three groups, 10 mice per group. Zn-normal diet group (ZN group) was given Zn-normal diet（Zn content= 30 mg/kg）for 8 weeks. Zn-deficienct diet group (ZD group) was given Zn-deficienct diet（Zn content< 1 mg/kg）for 8 weeks. Zn-deficient and Zn-normal diet group（ZDN group）was given 4 weeks Zn-deficienct diet followed by 4 weeks Zn-normal diet. After 8 weeks, the overnight fasted mice were sacrificed, and blood and organs were collected for further analysis.ResultsThe experimental results showed that Zn-deficienct diet leads to increased abnormal morphology sperm and testicular oxidative stress.The rate of abnormal morphology sperm, chromomycin A3(CMA3), DNA fragmentation index (DFI), malondialdehyde (MDA) were significantly increased, and a-kinase anchor protein 4(AKAP4), dynein axonemal heavy chain 1(DNAH1), sperm associated antigen 6(SPAG6), cilia and flagella associated protein 44(CFAP44), glutathione peroxidase (GSH-PX), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), nuclear factor erythroid 2-related factor (NRF2), NAD(P)H:quinone oxidoreductase 1(NQO1)and heme oxygenase 1(HO1) were significantly decreased in the ZD group mice. While the changes in above indicators caused by Zn-deficient diet were significantly alleviated in the ZDN group.ConclusionIt was concluded that Zn-deficient diet causes abnormal morphology sperm and testicular oxidative stress in male mice. Abnormal morphology sperm caused by Zn-deficient diet are reversible, and Zn-normal diet can alleviate them. |
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| Keywords: | Zinc Sperm Sperm morphology Oxidative stress |
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