Purification and evaluation of 2, 4-di-tert butylphenol (DTBP) as a biocontrol agent against phyto-pathogenic fungi |
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| Institution: | 1. Department of Biotechnology, School of Life Sciences, Pondicherry University, Pondicherry, India;2. Department of Bioinformatics, School of Life Sciences, Pondicherry University, Pondicherry, India;1. Department of Biological Sciences, Faculty of Science, University of Botswana, Private Bag 0022, Gaborone, Botswana;2. School of Allied Health Professions, Faculty of Health Sciences, University of Botswana, Private Bag 0022, Gaborone, Botswana;1. Université de Reims Champagne-Ardenne, INRAE, FARE, UMR A 614, AFERE, Reims, France;2. Université de Reims Champagne-Ardenne, INRAE, RIBP USC 1488, SFR Condorcet, Reims, France;1. Centro Avançado de Pesquisas em Proteção Plantas e Saúde Animal, Instituto Biológico, Alameda dos Vidoeiros, Campinas, São Paulo, Brazil;2. Instituto Biológico, Avenida Conselheiro Rodrigues Alves, São Paulo, Brazil;3. Department of Biology, Faculty of Arts and Science, Aydin Adnan Menderes University, Aydin, Turkey;1. Laboratorio de Salud de Bosques, Instituto de Conservación, Biodiversidad y Territorio, Facultad de Ciencias Forestales y Recursos Naturales, Universidad Austral de Chile, Valdivia, Chile;2. Laboratorio de Biodiversidad y Ecología Del Dosel, Instituto de Conservación, Biodiversidad y Territorio, Facultad de Ciencias Forestales y Recursos Naturales, Universidad Austral de Chile, Valdivia, Chile;3. Departamento de Ciencias Biológicas y Biodiversidad, Universidad de Los Lagos, Av. Fuschlöcher, 1305, Osorno, Chile;4. Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brazil;5. USDA-ARS Emerging Pests and Pathogens Research Unit, Robert W. Holley Center for Agriculture and Health, Ithaca, NY, USA |
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| Abstract: | A fungal strain, Marasmiellus sp (PUK64), isolated from the mangrove forests in Muthupet, Tamil Nadu, East coast of India, along with others were screened for the search of potent bioactive compounds. A phenolic compound, 2,4-di-tert-butylphenol (DTBP), was isolated from the most promising strain PUK64 and its chemical structure was ascertained. DTBP demonstrated remarkable antifungal activity against the phytopathogenic fungi Aspergillus oryzae, Curvularia lunata and Fusarium verticillioides. In an in-vitro experimental setup, DTBP suppressed the growth of all three fungi, among which F. verticillioides was found to be highly susceptible. This effect relates with the inhibition of spore germination and hyphal growth that we observed. DTBP showed high affinity with the F. verticillioides's β-tubulin protein (determined by ligand-protein docking) as compared to the standard fungicide carbendazim (CBZ). Molecular docking and simulation studies of DTBP with target β-tubulin further confirmed the potential of β-tubulin binding in F. verticillioides. To our knowledge, this is the first report on DTBP-mediated biocontrol of phytopathogenic fungi, produced by Marasmiellus sp. PUK64 that can be potent inhibitor of β-tubulin protein of F. verticillioides. |
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| Keywords: | 2 4-di-tert-butyl-phenol (DTBP) Biocontrol β-tubulin Docking-simulation |
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