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Controlling genetic heterogeneity in gene-edited hematopoietic stem cells by single-cell expansion
Institution:1. Laboratory for Stem Cell Therapy, Faculty of Medicine, Tsukuba University, Tsukuba 305-8577, Japan;2. Division of Stem Cell Biology, Center for Stem Cell Therapy, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan;3. MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, UK;4. Division of Malaria Immunology and International Vaccine Design Center, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan;5. Department of Pathology, Research Hospital, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan;6. International Research Center for Medical Sciences, Kumamoto University, Kumamoto City 860-0811, Japan;7. Pharma Solutions, Nutrition & Health, BASF SE, Carl-Bosch-Strasse 38, 67056 Ludwigshafen am Rhein, Germany;8. Pharma Solutions, Nutrition & Health, BASF Japan Ltd, Tokyo 103-0022, Japan;9. Tokyo Medical and Dental University, Tokyo 113-8510, Japan;10. York Biomedical Research Institute, Department of Biology, University of York, Wentworth Way, York YO10 5DD, UK
Abstract:
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  • Keywords:hematopoietic stem cell  clonal expansion  chemically defined culture  stem cell culture  gene editing  CRISPR-Cas9  transplantation  regenerative medicine  gene therapy
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