A reduction in temperature induces bioactive red pigment production in a psychrotolerant Penicillium sp. GEU_37 isolated from Himalayan soil |
| |
| Institution: | 1. Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, 248002, Uttarakhand, India;2. Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, 176061, India;3. Department of Genome Sciences, University of Washington, Seattle, WA, 98195, USA;4. National Centre for Cell Science, Pune, Maharashtra, 411007, India;1. DBT-National Centre for Cell Science, Pune, Maharashtra, 411007, India;2. Byramjee Jeejeebhoy Government Medical College, Pune, Maharashtra, 411001, India;1. Programa de Pós-Graduação em Microbiologia e Parasitologia, Laboratório de Micologia, Departamento de Microbiologia e Parasitologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil;2. Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil;3. Programa de Pós-Graduação em Microbiologia Agrícola e do Ambiente, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil;4. Laboratório Regional de Diagnóstico, Universidade Federal de Pelotas, Pelotas, RS, Brazil;1. Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, 176061, Himachal Pradesh, India;2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, Uttar Pradesh, India;3. Graphic Era Deemed to be University, Dehradun, 248002, Uttarakhand, India;1. Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun, 130033, Jilin Province, PR China;2. Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, 100193, PR China;1. Mund-Lagowski Department of Chemistry and Biochemistry, Bradley University, 1501 West Bradley Avenue, Peoria, IL, 61625, USA;2. USDA, Agricultural Research Service, National Center for Agricultural Utilization Research, Mycotoxin Prevention and Applied Microbiology Research Unit, 1815 N University St., Peoria, IL, 61604, USA;1. College of Food Science and Engineering, Bohai University, Jinzhou, 121013, China;2. National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, 121013, China |
| |
| Abstract: | Filamentous fungi are being globally explored for the production of industrially important bioactive compounds including pigments. In the present study, a cold and pH tolerant fungus strain Penicillium sp (GEU_37), isolated from the soil of Indian Himalaya, is characterized for the production of natural pigments as influenced by varying temperature conditions. The fungal strain produces a higher sporulation, exudation, and red diffusible pigment in Potato Dextrose (PD) at 15 °C as compared to 25 °C. In PD broth, a yellow pigment was observed at 25 °C. While measuring the effect of temperature and pH on red pigment production by GEU_37, 15 °C and pH 5, respectively, were observed to be the optimum conditions. Similarly, the effect of exogenous carbon and nitrogen sources and mineral salts on pigment production by GEU_37 was assessed in PD broth. However, no significant enhancement in pigmentation was observed. Chloroform extracted pigment was separated using thin layer chromatography (TLC) and column chromatography. The two separated fractions i.e., fractions I and II with Rf values 0.82 and 0.73, exhibited maximum light absorption, λmax, at 360 nm and 510 nm, respectively. Characterization of pigments using GC–MS showed the presence of the compounds such as phenol, 2,4-bis (1,1-dimethylethyl) and eicosene from fraction I and derivatives of coumarine, friedooleanan, and stigmasterole in fraction II. However, LC-MS analysis detected the presence of derivatives of compound carotenoids from fraction II as well as derivative of chromenone and hydroxyquinoline as major compounds from both the fractions along with other numerous important bioactive compounds. The production of such bioactive pigments under low temperature conditions suggest their strategic role in ecological resilience by the fungal strain and may have biotechnological applications. |
| |
| Keywords: | Low temperature Bioactive pigments Column chromatography TLC GC–MS and LC-MS Ecological resilience |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
