A secondary function of trehalose-6-phosphate synthase is required for resistance to oxidative and desiccation stress in Fusarium verticillioides |
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| Affiliation: | 1. Mund-Lagowski Department of Chemistry and Biochemistry, Bradley University, 1501 West Bradley Avenue, Peoria, IL, 61625, USA;2. USDA, Agricultural Research Service, National Center for Agricultural Utilization Research, Mycotoxin Prevention and Applied Microbiology Research Unit, 1815 N University St., Peoria, IL, 61604, USA;1. Centro Avançado de Pesquisas em Proteção Plantas e Saúde Animal, Instituto Biológico, Alameda dos Vidoeiros, Campinas, São Paulo, Brazil;2. Instituto Biológico, Avenida Conselheiro Rodrigues Alves, São Paulo, Brazil;3. Department of Biology, Faculty of Arts and Science, Aydin Adnan Menderes University, Aydin, Turkey;1. Laboratorio de Salud de Bosques, Instituto de Conservación, Biodiversidad y Territorio, Facultad de Ciencias Forestales y Recursos Naturales, Universidad Austral de Chile, Valdivia, Chile;2. Laboratorio de Biodiversidad y Ecología Del Dosel, Instituto de Conservación, Biodiversidad y Territorio, Facultad de Ciencias Forestales y Recursos Naturales, Universidad Austral de Chile, Valdivia, Chile;3. Departamento de Ciencias Biológicas y Biodiversidad, Universidad de Los Lagos, Av. Fuschlöcher, 1305, Osorno, Chile;4. Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brazil;5. USDA-ARS Emerging Pests and Pathogens Research Unit, Robert W. Holley Center for Agriculture and Health, Ithaca, NY, USA;1. Centre for Microbial Ecology and Genomics, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria, South Africa;2. Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Chile;3. Gobabeb-Namib Research Institute, Walvis Bay, Namibia;1. Université de Reims Champagne-Ardenne, INRAE, FARE, UMR A 614, AFERE, Reims, France;2. Université de Reims Champagne-Ardenne, INRAE, RIBP USC 1488, SFR Condorcet, Reims, France |
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| Abstract: | The disaccharide trehalose has long been recognized for its role as a stress solute, but in recent years some of the protective effects previously ascribed to trehalose have been suggested to arise from a function of the trehalose biosynthesis enzyme trehalose-6-phosphate (T6P) synthase that is distinct from its catalytic activity. In this study, we use the maize pathogenic fungus Fusarium verticillioides as a model to explore the relative contributions of trehalose itself and a putative secondary function of T6P synthase in protection against stress as well as to understand why, as shown in a previous study, deletion of the TPS1 gene coding for T6P synthase reduces pathogenicity against maize. We report that a TPS1-deletion mutant of F. verticillioides is compromised in its ability to withstand exposure to oxidative stress meant to simulate the oxidative burst phase of maize defense and experiences more ROS-induced lipid damage than the wild-type strain. Eliminating T6P synthase expression also reduces resistance to desiccation, but not resistance to phenolic acids. Expression of catalytically-inactive T6P synthase in the TPS1-deletion mutant leads to a partial rescue of the oxidative and desiccation stress-sensitive phenotypes, suggesting the importance of a T6P synthase function that is independent of its role in trehalose synthesis. |
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| Keywords: | T6P synthase Filamentous fungus Lipid peroxidation Phenolic acids Fumonisins |
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