Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada. |
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Authors: | K Angelis J Bríza J Satava I Skákal J Velemínsky J Vlasák K Kleibl G P Margison |
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Affiliation: | Institute of Experimental Botany CSAV, Prague. |
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Abstract: | The protein coding region of the E. coli gene ada has been transferred to tobacco plants by a leaf disc transformation procedure involving an Agrobacterium tumefaciens Ti plasmid. Transformed plants were shown to be transgenic for the ada message and had increased levels of O6-alkylguanine DNA alkyltransferase activity. The N-methyl-N-nitrosourea- or taurinechlorethylnitrosourea-induced inhibition of growth of calluses or of cells in suspension was considerably lower in ada-transformed than in non-transformed plants. This indicates that O6-alkylguanine, O4-alkylthymine or phosphotriesters are growth-inhibitory lesions in tobacco. |
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