Biological control of Phytophthora blight in red pepper (Capsicum annuum L.) using Bacillus
subtilis |
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Authors: | Kui Jae Lee Seralathan Kamala-Kannan Han Sang Sub Cho Kyu Seong Gun Woong Lee |
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Institution: | (1) Division of Bioresources Science, College of Agriculture and Life Science, Chonbuk National University, Jeonju, 561-756, South Korea;(2) Research Institute of Bioindustry, College of Agriculture and Life Science, Chonbuk National University, Jeonju, 561-756, South Korea;(3) Division of Forest Science, College of Agriculture and Life Science, Chonbuk National University, Jeonju, 561-756, South Korea;(4) Division of Science Education, Chonbuk National University, Jeonju, 561-756, South Korea |
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Abstract: | Phytophthora blight is one of the most important devastating diseases of red pepper plants. Forty-one bacterial isolates were obtained from rhizosphere soil and subsequently tested for antagonistic activity under
in vitro and in vivo conditions. Among the 41 isolates tested, 12 exhibited a maximum antagonistic activity in dual culture
assay. These 12 isolates were further screened for disease suppression on red pepper plants in both natural and greenhouse
conditions. All the antagonists showed varying levels of antagonism, whereas the isolates R33 and R13 exhibited the maximum
(86.8 and 71%) ability to reduce the disease severity in in vivo conditions. Based on the 16S rDNA sequencing, the most effective
isolates were identified as Bacillus
subtilis. In addition, the isolates were also screened for siderophores, hydrogen cyanide and hydrolytic enzymes. Further, the isolates
increased the root and shoot length of the red pepper, which is an added advantage of the isolates while performing the desired
function. |
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Keywords: | Bacillus subtilis Biological control Phytophthora capsici Red pepper Rhizobacteria |
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