Conformation and length of the signal sequence affect processing of secretory protein. |
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| Authors: | A Kohara Y Yamamoto M Kikuchi |
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| Affiliation: | Protein Engineering Research Institute, Osaka, Japan. |
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| Abstract: | Processing of human lysozyme with artificially designed signal sequences was examined in an in vitro translation-translocation system and compared with their secretory capabilities in yeast. It has been shown that the conformation of the C-terminal region of the signal sequence and the length of the hydrophobic segment are important factors for efficient cleavage of the signal sequence. |
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