Exchange of Domain I from Bacillus thuringiensis Cry1 Toxins Influences Protoxin Stability and Crystal Formation |
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| Authors: | Cécile Rang Vincent Vachon Florence Coux Céline Carret William J Moar Roland Brousseau Jean-Louis Schwartz Raynald Laprade Roger Frutos |
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| Institution: | (1) CIRAD, TA 40/PS1, Boulevard de la Lironde, 34398 Montpellier Cedex 5, France, FR;(2) Groupe de recherche en transport membranaire, Université de Montréal, P.O. Box 6128, Centre Ville Station, Montreal, Quebec H3C 2J7, Canada, CA;(3) Department of Entomology, 301 Funchess Hall, Auburn University, Auburn, AL 36849, USA, US;(4) Biotechnology Research Institute, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2, Canada, CA |
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| Abstract: | Influence of domain I exchange on the stability and production of Bacillus thuringiensis Cry1 protoxins as well as on the shape of inclusion and toxicity to Spodoptera exigua and Plutella xylostella larvae was investigated. Chimeric genes were prepared by exchanging the regions coding for domain I between Cry1Aa, Cry1Ab,
Cry1Ac, Cry1C, and Cry1E. The AcCC chimera accumulated into bipyramidal inclusion bodies, whereas CEE produced round-shaped
inclusion bodies, and ECC and AaEE protoxins produced small granules. AbEE and EAaAa did not produce any inclusion body and
were visualized by immunodetection only. AcCC, CEE, ECC, and AaEE were stable to trypsin, whereas AbEE and EAaAa were not.
Bioassays showed that the chimeras were not toxic in vivo. However, S. exigua larvae fed with the activated AcCC toxin displayed a lower growth rate.
Received: 14 October 2000 / Accepted: 17 November 2000 |
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