Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation |
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Authors: | Latiporn Udomsuk Kanokwan Jarukamjorn Hiroyuki Tanaka Waraporn Putalun |
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Affiliation: | (1) Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand;(2) Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan; |
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Abstract: | The effect of abiotic and biotic elicitors (methyl jasmonate, chitosan, salicylic acid, Agrobacterium, and yeast extract) at various concentrations on total isoflavonoid accumulation was studied in the hairy root cultures of Pueraria candollei. All elicitors stimulated isoflavonoid production. Yeast extract (0.5 mg/ml) was the most efficient giving total isoflavonoids at 60 ± 1 mg/g dry wt, which was 4.5-fold higher than control hairy roots on day 3 of elicitation. |
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