Oxygenation capabilities of basket-type bioreactors for microcarrier cultures of anchorage-dependent cells |
| |
| Authors: | N. Kalogerakis L. A. Behie |
| |
| Affiliation: | Bioengineering Laboratory, Department of Chemical Engineering, State University of New York, Buffalo, NY 14260–4200,
Pharmaceutical Production Research Facility, Faculty of Engineering, The University of Calgary, Calgary, Alberta T2N 1N4,
|
| |
| Abstract: | The oxygenation capabilities of a new generation three phase – two region 1200 l bioreactor employed for the cultivation of anchorage dependent animal cells were investigated experimentally. A mathematical model has also been developed that explains qualitatively the observed oxygenation characteristics. This type of bioreactor, that uses microcarrier support particles, has two distinct mesh-separated regions so that the air bubbles in the oxygenation region do not come into contact with the microcarriers in the cell (bubble-free) region. Implications on achievable maximum cell densities are also discussed. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
