hSHIP induces S-phase arrest and growth inhibition in cervical cancer HeLa cells |
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| Authors: | Kangxia He b Jie He b Shengyu Wang a Jianghua Yan a a Cancer Research Center Medical College Xiamen University Siming South Road Xiamen China b School of Life Science China |
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| Institution: | Kangxia He b,Jie He b,Shengyu Wang a,Jianghua Yan a,a Cancer Research Center,Medical College,Xiamen University,422 Siming South Road,Xiamen 361005,China b School of Life Science,China |
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| Abstract: | hSHIP,a human SH2-containing inositol-5-phosphatase,acts as a negative regulator of proliferation and survival in hematopoietic cells.Therefore,hSHIP may play a crucial role in suppression of cervical cancer HeLa cells.In this study,pcDNA3.1-hSHIP-GFP plasmid was constructed and transfected into HeLa cells with Lipofectamine2000,stably transfected HeLa cells were established and their responses were investigated by Flow cytometry,MTT,tumorigenicity in nude mice,RT-PCR and ELISA assays.The results showed tha... |
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| Keywords: | SH2-containing inositol-5-phosphatase(SHIP) cell cycle cell growth PI3K/Akt HeLa cells |
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