Peculiarities of dopamine receptors on the membrane of multipolar spinal cord neurons of the brook lamprey <Emphasis Type="Italic">Lampetra planeri</Emphasis> |
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Authors: | A A Bukinich E A Tsvetkov N P Vesselkin |
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Institution: | (1) Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia |
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Abstract: | On isolated multipolar neurons of spinal cord of amniocoete (larva of the brook lamprey Lampetra planeri) by the patch-clamp method in configuration “the whole cell,” a modulating effect of dopamine on potential-activated Na+ currents was studied. Application of dopamine (10 μM) was shown to produce a complex action on the sodium current amplitude.
In some cases a decrease of the amplitude, on average, by 13.5 ± 2.2% was found, while in others—an increase, on average,
by 8.6 ± 6.1%. The modulation dopamine effect was not accompanied by any changes either of the threshold of the current appearance
or of resistance of neuronal cell membranes. Pharmacological analysis with use of dopamine agonist has shown that the agonist
of D1-receptors (−)-SKF-38393 (10 μM) decreases the Na+ current amplitude, whereas the agonist of D2-receptors (−)-quinpirole
(10 μM) can produce in different cells both an increase, by 30.7 ± 17.0%, and a decrease, by 13.2 ± 3.1%, of the Na+ current
amplitude. The obtained data indicate the existence of D1-and D2-receptors on the membrane of multipolar spinal neurons of
the amniocoete (larva of the brook lamprey). Study of action of antagonists has shown that the antagonist of D1-receptors
(+)-SCH-23390 (10 μM) does not affect action of the agonist of D1-receptors (−)-SKF-38393 (10 μM); the antagonist of D2-receptors
(−)-sulpiride (10 μM) blocks completely effects both of the agonist of D1-receptors (−)-SKF-38393 (10 μM) and of the agonist
of D2-receptors (−)-quinpirole (10 μM). The antagonist of D1-receptors (+)-SCH-23390 (10 μM) produced no effect on action
of the agonist of D1-receptors (−)-SKF-38393 (10 μM). The obtained data indicate peculiarities of dopamine receptors of Cyclostomata
as compared with those in mammals.
Original Russian Text ? A. A. Bukinich, E. A. Tsvetkov, and N. P. Vesselkin, 2007, published in Zhurnal Evolyutsionnoi Biokhimii
i Fiziologii, 2007, Vol. 43, No. 1, pp. 39–45. |
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