Knockdown of KLF9 promotes the differentiation of both intramuscular and subcutaneous preadipocytes in goat |
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| Authors: | Qing Xu Yong Wang Wenlin Bai Jiangjiang Zhu |
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| Institution: | 1. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education , Chengdu, China;2. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization , Sichuan Province, Chengdu, China;3. School of Life Science and Technology, Southwest Minzu University , Chengdu, China;4. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization , Sichuan Province, Chengdu, China;5. College of Animal Science &6. Veterinary Medicine, Shenyang Agricultural University , Shenyang, China |
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| Abstract: | ABSTRACT KLF9 is reported to promote adipocyte differentiation in 3T3-L1 cells and pigs. However, the roles of KLF9 in adipocytes differentiation of goat remain unknown. In this study, the expression profiles of KLF9 were different between subcutaneous and intramuscular preadipocytes of goat during differentiation process. After silencing KLF9 gene, the lipid droplets were increased in both two types of adipocytes. In subcutaneous preadipocyte with silencing KLF9, the expressions of C/EBPβ, PPARγ, LPL, KLF1-2, KLF5, and KLF17 genes were up-regulated, while KLF12, KLF4, and KLF13 genes were down-regulated in expression level. In intramuscular preadipocyte, aP2, C/EBPα, KLF2-3, KLF5, and KLF7 gene were up-regulated, and Pref-1 gene was down-regulated. In addition, the binding sites of KLF9 existed in the promoters of aP2, C/EBPα, C/EBPβ, LPL and Pref-1. Taken together, KLF9 play a negative role in the differentiation of both intramuscular and subcutaneous preadipocytes in goats, but the functional mechanism may be different. |
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| Keywords: | Goat KLF9 siRNA interference subcutaneous adipocytes intramuscular adipocytes |
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