A fluorescence method to visualize the nuclear boundary by the lipophilic dye DiI |
| |
| Authors: | Kohei Miyazaki Ken-Ichi Yano |
| |
| Institution: | 1. Department of Biological Science, Graduate School of Science and Technology, Kumamoto University , Kumamoto, Japan;2. Institute of Pulsed Power Science, Kumamoto University , Kumamoto, Japan |
| |
| Abstract: | ABSTRACT Here, we describe a procedure to fluorescently contrast the nuclear boundary using the lipophilic carbocyanine dye DiI in cultured human cells. Our procedure is simple and is applicable to detect nuclear boundary defects, which may be relevant to studies on nuclear envelope dynamics, micronuclei formation and cancer biology. |
| |
| Keywords: | Cancer lipophilic carbocyanine dye lipid membrane micronucleus nuclear envelope |
|
|
